- Undergraduate Education
- B.S. (2004) Physics, Fudan University
- Graduate Education
- Ph.D. (2009) Structural Biology, Baylor College of Medicine
- Postdoc. Structural Biology, Stanford University (2009-13)
- Joined Texas A&M in January 1, 2014
Cryo-EM of Cellular Machineries
The living cell contains a collection of molecular machines to grow and function. These machines include the ribosomes, the chaperons, the proteasomes and other enzymes. Malfunction of these machines, if occurred in human, are related to many diseases. Understanding their three-dimensional (3D) structures is essential to understand how these machines work in the cell and eventually to treat those related diseases.
Here we use an experimental technique called cryo-electron microscopy (cryo-EM) to image these cellular machines in their native environment at liquid nitrogen temperatures. We then use image processing and graphics techniques to visualize their 3D structures, answering the questions such as how they assemble and how they interact with each other.
In addition, we develop computational modeling tools to interpret and animate these obtained 3D structures to further describe their movements and dynamics.
Théry, C, Witwer, KW, Aikawa, E, Alcaraz, MJ, Anderson, JD, Andriantsitohaina, R et al.. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2018;7 (1):1535750.
Zhang, J, Tan, P, Guo, L, Gong, J, Ma, J, Li, J et al.. p53-dependent autophagic degradation of TET2 modulates cancer therapeutic resistance. Oncogene. 2019;38 (11):1905-1919.
Klein, BJ, Vann, KR, Andrews, FH, Wang, WW, Zhang, J, Zhang, Y et al.. Structural insights into the π-π-π stacking mechanism and DNA-binding activity of the YEATS domain. Nat Commun. 2018;9 (1):4574.
Brock, DJ, Kustigian, L, Jiang, M, Graham, K, Wang, TY, Erazo-Oliveras, A et al.. Efficient cell delivery mediated by lipid-specific endosomal escape of supercharged branched peptides. Traffic. 2018;19 (6):421-435.
Cui, Z, Gorzelnik, KV, Chang, JY, Langlais, C, Jakana, J, Young, R et al.. Structures of Qβ virions, virus-like particles, and the Qβ-MurA complex reveal internal coat proteins and the mechanism of host lysis. Proc. Natl. Acad. Sci. U.S.A. 2017;114 (44):11697-11702.
Yang, K, Chang, JY, Cui, Z, Li, X, Meng, R, Duan, L et al.. Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis. Nucleic Acids Res. 2017;45 (18):10884-10894.
Weaver, J, Jiang, M, Roth, A, Puchalla, J, Zhang, J, Rye, HS et al.. GroEL actively stimulates folding of the endogenous substrate protein PepQ. Nat Commun. 2017;8 :15934.
Gorzelnik, KV, Cui, Z, Reed, CA, Jakana, J, Young, R, Zhang, J et al.. Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ. Proc. Natl. Acad. Sci. U.S.A. 2016;113 (41):11519-11524.
Yang, S, Jiang, M, Grabowska, MM, Li, J, Connelly, ZM, Zhang, J et al.. Androgen receptor differentially regulates the proliferation of prostatic epithelial cells in vitro and in vivo. Oncotarget. 2016;7 (43):70404-70419.
Lee, JR, Xie, X, Yang, K, Zhang, J, Lee, SY, Shippen, DE et al.. Dynamic Interactions of Arabidopsis TEN1: Stabilizing Telomeres in Response to Heat Stress. Plant Cell. 2016;28 (9):2212-2224.