- Undergraduate Education
- A.B. Rice University (1968)
- Graduate Education
- Ph.D. University of Texas, Dallas (1975)
- Postdoc. Harvard Medical School (1975-78)
- Joined Texas A&M in 1978
The Molecules and Mechanisms of Bacteriophage Lysis
Most bacterial viruses (phages) cause lysis of their host cell to release the progeny virions. Large phages elaborate an enzyme (“endolysin”) to degrade the cell wall and also a small membrane protein (“holin”). The holin accumulates in the membrane and then, at a precisely scheduled time, suddenly forms a hole to allow release of endolysin through the cytoplasmic membrane to gain access to the wall. We use molecular genetics and biochemistry to study how this small protein is able to act as a molecular “clock” and punch holes in membranes. Small phages make single proteins which cause host lysis in a different way. This strategy is to target the host cell wall synthesis machinery; that is, the virus makes a “protein antibiotic” that causes lysis in the same way as antibiotics like penicillin by inhibiting an enzyme in the multi-step pathway of murein biosynthesis. Thus, when the infected cell tries to divide, it blows up, or lyses, because it can’t make the new cell wall between the daughter cells. Remarkably, each of three different, small phages blocks a different step in the pathway. These small lysis proteins are models for a completely new class of antibacterial antibiotics. Also, the E. coli SlyD protein is required for this mode of lysis in one case. SlyD is a member of an ubiquitous family of proteins related to human “immunophilins,” the targets of immune-suppression drugs. We study SlyD to learn about the role of this class of proteins in biology.
Chamakura, KR, Sham, LT, Davis, RM, Min, L, Cho, H, Ruiz, N et al.. A viral protein antibiotic inhibits lipid II flippase activity. Nat Microbiol. 2017; :.
Schooley, RT, Biswas, B, Gill, JJ, Hernandez-Morales, A, Lancaster, J, Lessor, L et al.. Development and use of personalized bacteriophage-based therapeutic cocktails to treat a patient with a disseminated resistant Acinetobacter baumannii infection. Antimicrob. Agents Chemother. 2017; :.
Chamakura, KR, Edwards, GB, Young, R. Mutational analysis of the MS2 lysis protein L. Microbiology (Reading, Engl.). 2017;163 (7):961-969.
Piya, D, Vara, L, Russell, WK, Young, R, Gill, JJ. The multicomponent antirestriction system of phage P1 is linked to capsid morphogenesis. Mol. Microbiol. 2017;105 (3):399-412.
Cahill, J, Rajaure, M, Holt, A, Moreland, R, O'Leary, C, Kulkarni, A et al.. Suppressor Analysis of the Fusogenic Lambda Spanins. J. Virol. 2017;91 (14):.
Chamakura, KR, Tran, JS, Young, R. MS2 Lysis of Escherichia coli Depends on Host Chaperone DnaJ. J. Bacteriol. 2017;199 (12):.
Yao, GW, Duarte, I, Le, TT, Carmody, L, LiPuma, JJ, Young, R et al.. A Broad-Host-Range Tailocin from Burkholderia cenocepacia. Appl. Environ. Microbiol. 2017;83 (10):.
Cahill, J, Rajaure, M, O'Leary, C, Sloan, J, Marrufo, A, Holt, A et al.. Genetic Analysis of the Lambda Spanins Rz and Rz1: Identification of Functional Domains. G3 (Bethesda). 2017;7 (2):741-753.
Gorzelnik, KV, Cui, Z, Reed, CA, Jakana, J, Young, R, Zhang, J et al.. Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ. Proc. Natl. Acad. Sci. U.S.A. 2016;113 (41):11519-11524.
Chen, Y, Young, R. The Last r Locus Unveiled: T4 RIII Is a Cytoplasmic Antiholin. J. Bacteriol. 2016;198 (18):2448-57.