- Undergraduate Education
- B.A. Lewis & Clark College, 1992
- Graduate Education
- Ph.D. University of Colorado, Boulder, 2000
- Postdoc. Harvard Medical School, 2001-2007
- Joined Texas A&M in 2008
- NSF CAREER Award 2013
Microbial Interactions and Secondary Metabolism
Microorganisms produce the majority of our antibiotics, antifungal, antiviral, and anticancer therapeutics. Yet, these natural product medicines represent only a small fraction of the chemically diverse small molecules produced primarily by bacteria and fungi. The functions of such compounds in microbial communities are sure to be as diverse as the chemical structures themselves, although little is known beyond a handful of examples. Our laboratory studies the function of small molecules as chemical signals in the microbial world. We focus on the interactions between different species of bacteria in co-culture to understand how diverse chemical signals are synthesized and perceived.
Our goal is to understand how microorganisms interact in complex communities. Specifically, we study how small molecules produced in a microbial community affect the growth, development and metabolic output of the organisms. We use a combination of microbiology, genetic, genomic, and biochemical approaches to dissect complex interspecies interactions. Currently, our research focuses on the interactions of the soil bacteria Bacillus subtilis and members of the genus Streptomyces, known for their prolific production of bioactive small molecules and development of aerial structures and spores.
Microbial developmental processes are particularly attuned to environmental signals, including those generated by neighboring organisms. In one area of research interest, we investigate developmental interactions between bacteria in order to probe the mode of action of small molecules beyond a query of antibiotic activity. As a second area of interest, we study the regulation and assembly of the enzymatic machinery for small molecule synthesis and export by the producer cell. To synthesize low molecular weight compounds of precise chemical structure, microorganisms use protein enzymatic complexes that are often larger than the ribosome. The energy invested in producing these synthases underscores the importance of the compounds in environmentally relevant settings. Understanding the activity and synthesis of microbial small molecules will help us predict the outcome of complex interactions in natural environments and will highlight strategies for discovery of new therapeutics and new therapeutic targets.
Zhang, C, Straight, PD. Antibiotic discovery through microbial interactions. Curr. Opin. Microbiol. 2019;51 :64-71.
Liu, Y, Kyle, S, Straight, PD. Antibiotic Stimulation of a Bacillus subtilis Migratory Response. mSphere. ;3 (1):.
Stubbendieck, RM, Brock, DJ, Pellois, JP, Gill, JJ, Straight, PD. Linearmycins are lytic membrane-targeting antibiotics. J. Antibiot. 2018;71 (3):372-381.
Hoefler, BC, Stubbendieck, RM, Josyula, NK, Moisan, SM, Schulze, EM, Straight, PD et al.. A Link between Linearmycin Biosynthesis and Extracellular Vesicle Genesis Connects Specialized Metabolism and Bacterial Membrane Physiology. Cell Chem Biol. 2017;24 (10):1238-1249.e7.
Stubbendieck, RM, Straight, PD. Linearmycins Activate a Two-Component Signaling System Involved in Bacterial Competition and Biofilm Morphology. J. Bacteriol. 2017;199 (18):.
Goodson, JR, Klupt, S, Zhang, C, Straight, P, Winkler, WC. LoaP is a broadly conserved antiterminator protein that regulates antibiotic gene clusters in Bacillus amyloliquefaciens. Nat Microbiol. 2017;2 :17003.
Stubbendieck, RM, Vargas-Bautista, C, Straight, PD. Bacterial Communities: Interactions to Scale. Front Microbiol. 2016;7 :1234.
Stubbendieck, RM, Straight, PD. Multifaceted Interfaces of Bacterial Competition. J. Bacteriol. 2016;198 (16):2145-55.
Stubbendieck, RM, Straight, PD. Correction: Escape from Lethal Bacterial Competition through Coupled Activation of Antibiotic Resistance and a Mobilized Subpopulation. PLoS Genet. 2016;12 (1):e1005807.
Stubbendieck, RM, Straight, PD. Escape from Lethal Bacterial Competition through Coupled Activation of Antibiotic Resistance and a Mobilized Subpopulation. PLoS Genet. 2015;11 (12):e1005722.