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Dorothy Shippen

Shippen, Dorothy
Dorothy Shippen
Professor
Office:
BioBio / Room 413A
Email:
Phone:
979-862-2342
http://www.planttelomeres.com
Undergraduate Education
B.S. Auburn University (1982)
Graduate Education
Ph.D. University of Alabama, Birmingham (1987)
Postdoc. University of California, Berkeley and University of California, San Francisco (1987-91)
Joined Texas A&M in 1991

Telomere Biology

We are taking biochemical, molecular genetic and cytological approaches to study the structure, function and maintenance of telomeres. Telomeres are higher order nucleoprotein complexes that cap the ends of eukaryotic chromosomes and play essential roles in conferring genome stability and cell proliferation capacity. The protective cap of the telomere is comprised of specific telomere binding proteins that regulate the length of telomeric DNA tract and allow the cell distinguish the chromosome terminus from a double-strand break. Telomeric DNA is synthesized by the action of telomerase, an unusual reverse transcriptase that replenishes telomeric DNA lost as a consequence of replication by conventional DNA polymerases. We have developed the genetically tractable flowering plant Arabidopsis thaliana as a model system for studying telomeres in higher eukaryotes. With its sequenced genome, abundant genetic and transgenic tools, and extraordinarily high tolerance to genome instability, Arabidopsis has proven to be an excellent model for investigating fundamental processes in telomere biology. Current studies focus on defining the function and molecular evolution of telomere capping proteins and components of the telomerase ribonucleoprotein complex.

Recent Publications

  1. Arora, A, Beilstein, MA, Shippen, DE. Evolution of Arabidopsis protection of telomeres 1 alters nucleic acid recognition and telomerase regulation. Nucleic Acids Res. 2016;44 (20):9821-9830.
    doi: 10.1093/nar/gkw807. PubMed PMID:27651456. PubMed Central PMC5175356.

  2. Lee, JR, Xie, X, Yang, K, Zhang, J, Lee, SY, Shippen, DE et al.. Dynamic interactions of Arabidopsis TEN1: stabilizing telomeres in response to heat stress. Plant Cell. 2016; :.
    doi: 10.1105/tpc.16.00408. PubMed PMID:27609839. PubMed Central PMC5059806.

  3. Nelson, AD, Shippen, DE. Evolution of TERT-interacting lncRNAs: expanding the regulatory landscape of telomerase. Front Genet. 2015;6 :277.
    doi: 10.3389/fgene.2015.00277. PubMed PMID:26442096. PubMed Central PMC4564757.

  4. Xu, H, Nelson, AD, Shippen, DE. A transposable element within the Non-canonical telomerase RNA of Arabidopsis thaliana modulates telomerase in response to DNA damage [corrected]. PLoS Genet. 2015;11 (6):e1005281.
    doi: 10.1371/journal.pgen.1005281. PubMed PMID:26075395. PubMed Central PMC4468102.

  5. González-García, MP, Pavelescu, I, Canela, A, Sevillano, X, Leehy, KA, Nelson, ADL et al.. Single-cell telomere-length quantification couples telomere length to meristem activity and stem cell development in Arabidopsis. Cell Rep. 2015;11 (6):977-989.
    doi: 10.1016/j.celrep.2015.04.013. PubMed PMID:25937286. PubMed Central PMC4827700.

  6. Beilstein, MA, Renfrew, KB, Song, X, Shakirov, EV, Zanis, MJ, Shippen, DE et al.. Evolution of the Telomere-Associated Protein POT1a in Arabidopsis thaliana Is Characterized by Positive Selection to Reinforce Protein-Protein Interaction. Mol. Biol. Evol. 2015;32 (5):1329-41.
    doi: 10.1093/molbev/msv025. PubMed PMID:25697340. PubMed Central PMC4408415.

  7. Renfrew, KB, Song, X, Lee, JR, Arora, A, Shippen, DE. POT1a and components of CST engage telomerase and regulate its activity in Arabidopsis. PLoS Genet. 2014;10 (10):e1004738.
    doi: 10.1371/journal.pgen.1004738. PubMed PMID:25329641. PubMed Central PMC4199523.

  8. Boltz, KA, Jasti, M, Townley, JM, Shippen, DE. Analysis of poly(ADP-Ribose) polymerases in Arabidopsis telomere biology. PLoS ONE. 2014;9 (2):e88872.
    doi: 10.1371/journal.pone.0088872. PubMed PMID:24551184. PubMed Central PMC3923816.

  9. Leehy, KA, Lee, JR, Song, X, Renfrew, KB, Shippen, DE. MERISTEM DISORGANIZATION1 encodes TEN1, an essential telomere protein that modulates telomerase processivity in Arabidopsis. Plant Cell. 2013;25 (4):1343-54.
    doi: 10.1105/tpc.112.107425. PubMed PMID:23572541. PubMed Central PMC3663272.

  10. Nelson, AD, Shippen, DE. Surprises from the chromosome front: lessons from Arabidopsis on telomeres and telomerase. Cold Spring Harb. Symp. Quant. Biol. 2012;77 :7-15.
    doi: 10.1101/sqb.2013.77.017053. PubMed PMID:23460576. PubMed Central PMC3836370.

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