- Graduate Education
- M.Sc. Ecole Supérieure de Chimie Physique Electronique de Lyon (1999)
- Ph.D. University of Houston (2002)
- Postdoc. The Rockefeller University (2002-2006)
- Joined Texas A&M in 2006
Protein Chemistry and Cell Biology
Research in the Pellois lab is currently focused on the following topics:
- development of reagents that can deliver macromolecules into live cells efficiently and safely
- use of such delivery approaches to deliver proteins into live cells so as to manipulate and investigate cellular processes
- development of peptide-based photosensitizers for the light-induced inactivation of drug-resistant bacteria
- elucidation of the mechanisms by which cells communicate with one another by sharing material via vesicle secretion and internalization
- mapping of intracellular protein-protein interactions and impact of viral protein mutations on virus/host responses
Each of these projects involves a combination of chemistry, biochemistry and cell biology. We typically investigate cellular processes at a molecular level and design new tools to study such processes. We use a wide variety of techniques, including protein engineering, peptide chemistry, molecular cloning, and live cell fluorescence microscopy.
Pellois, JP. Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays. Bioessays. 2019;41 (6):e1900031.
Brock, DJ, Kondow-McConaghy, HM, Hager, EC, Pellois, JP. Endosomal Escape and Cytosolic Penetration of Macromolecules Mediated by Synthetic Delivery Agents. Bioconjug. Chem. 2019;30 (2):293-304.
Fang, Y, Lian, X, Huang, Y, Fu, G, Xiao, Z, Wang, Q et al.. Investigating Subcellular Compartment Targeting Effect of Porous Coordination Cages for Enhancing Cancer Nanotherapy. Small. 2018;14 (47):e1802709.
Allen, JK, Brock, DJ, Kondow-McConaghy, HM, Pellois, JP. Efficient Delivery of Macromolecules into Human Cells by Improving the Endosomal Escape Activity of Cell-Penetrating Peptides: Lessons Learned from dfTAT and its Analogs. Biomolecules. 2018;8 (3):.
Brock, DJ, Kustigian, L, Jiang, M, Graham, K, Wang, TY, Erazo-Oliveras, A et al.. Efficient cell delivery mediated by lipid-specific endosomal escape of supercharged branched peptides. Traffic. 2018;19 (6):421-435.
Lian, X, Huang, Y, Zhu, Y, Fang, Y, Zhao, R, Joseph, E et al.. Enzyme-MOF Nanoreactor Activates Nontoxic Paracetamol for Cancer Therapy. Angew. Chem. Int. Ed. Engl. 2018;57 (20):5725-5730.
Stubbendieck, RM, Brock, DJ, Pellois, JP, Gill, JJ, Straight, PD. Linearmycins are lytic membrane-targeting antibiotics. J. Antibiot. 2018;71 (3):372-381.
Lian, X, Erazo-Oliveras, A, Pellois, JP, Zhou, HC. High efficiency and long-term intracellular activity of an enzymatic nanofactory based on metal-organic frameworks. Nat Commun. 2017;8 (1):2075.
Najjar, K, Erazo-Oliveras, A, Mosior, JW, Whitlock, MJ, Rostane, I, Cinclair, JM et al.. Unlocking Endosomal Entrapment with Supercharged Arginine-Rich Peptides. Bioconjug. Chem. 2017;28 (12):2932-2941.
Libardo, MDJ, Wang, TY, Pellois, JP, Angeles-Boza, AM. How Does Membrane Oxidation Affect Cell Delivery and Cell Killing? Trends Biotechnol. 2017;35 (8):686-690.