- Graduate Education
- M.Sc. Ecole Supérieure de Chimie Physique Electronique de Lyon (1999)
- Ph.D. University of Houston (2002)
- Postdoc. The Rockefeller University (2002-2006)
- Joined Texas A&M in 2006
Protein Chemistry and Cell Biology
Research in the Pellois lab is currently focused on the following topics:
- development of reagents that can deliver macromolecules into live cells efficiently and safely
- use of such delivery approaches to deliver proteins into live cells so as to manipulate and investigate cellular processes
- development of peptide-based photosensitizers for the light-induced inactivation of drug-resistant bacteria
- elucidation of the mechanisms by which cells communicate with one another by sharing material via vesicle secretion and internalization
- mapping of intracellular protein-protein interactions and impact of viral protein mutations on virus/host responses
Each of these projects involves a combination of chemistry, biochemistry and cell biology. We typically investigate cellular processes at a molecular level and design new tools to study such processes. We use a wide variety of techniques, including protein engineering, peptide chemistry, molecular cloning, and live cell fluorescence microscopy.
Diaz, J, Pellois, JP. Expressed Protein Ligation: General Experimental Protocols. Methods Mol. Biol. 2020;2133 :75-117.
Allen, J, Najjar, K, Erazo-Oliveras, A, Kondow-McConaghy, HM, Brock, DJ, Graham, K et al.. Cytosolic Delivery of Macromolecules in Live Human Cells Using the Combined Endosomal Escape Activities of a Small Molecule and Cell Penetrating Peptides. ACS Chem. Biol. 2019;14 (12):2641-2651.
Park, G, Brock, DJ, Pellois, JP, Gabbaï, FP. Heavy Pnictogenium Cations as Transmembrane Anion Transporters in Vesicles and Erythrocytes. Chem. 2019;5 (8):2215-2227.
Arora, M, Ganugula, R, Kumar, N, Kaur, G, Pellois, JP, Garg, P et al.. Next-Generation Noncompetitive Nanosystems Based on Gambogic Acid: In silico Identification of Transferrin Receptor Binding Sites, Regulatory Shelf Stability, and Their Preliminary Safety in Healthy Rodents. ACS Appl Bio Mater. 2019;2 (8):3540-3550.
Pellois, JP. Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays. Bioessays. 2019;41 (6):e1900031.
Brock, DJ, Kondow-McConaghy, HM, Hager, EC, Pellois, JP. Endosomal Escape and Cytosolic Penetration of Macromolecules Mediated by Synthetic Delivery Agents. Bioconjug. Chem. 2019;30 (2):293-304.
Fang, Y, Lian, X, Huang, Y, Fu, G, Xiao, Z, Wang, Q et al.. Investigating Subcellular Compartment Targeting Effect of Porous Coordination Cages for Enhancing Cancer Nanotherapy. Small. 2018;14 (47):e1802709.
Allen, JK, Brock, DJ, Kondow-McConaghy, HM, Pellois, JP. Efficient Delivery of Macromolecules into Human Cells by Improving the Endosomal Escape Activity of Cell-Penetrating Peptides: Lessons Learned from dfTAT and its Analogs. Biomolecules. 2018;8 (3):.
Brock, DJ, Kustigian, L, Jiang, M, Graham, K, Wang, TY, Erazo-Oliveras, A et al.. Efficient cell delivery mediated by lipid-specific endosomal escape of supercharged branched peptides. Traffic. 2018;19 (6):421-435.
Lian, X, Huang, Y, Zhu, Y, Fang, Y, Zhao, R, Joseph, E et al.. Enzyme-MOF Nanoreactor Activates Nontoxic Paracetamol for Cancer Therapy. Angew. Chem. Int. Ed. Engl. 2018;57 (20):5725-5730.