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Jean-Philippe Pellois

Pellois, Jean-Philippe
Jean-Philippe Pellois
Professor and Associate Head of Graduate Programs
BioBio / Room 438A
Graduate Education
M.Sc. Ecole Supérieure de Chimie Physique Electronique de Lyon (1999)
Ph.D. University of Houston (2002)
Postdoc. The Rockefeller University (2002-2006)
Joined Texas A&M in 2006

Protein Chemistry and Cell Biology

Research in the Pellois lab is currently focused on the following topics:

  • development of reagents that can deliver macromolecules into live cells efficiently and safely
  • use of such delivery approaches to deliver proteins into live cells so as to manipulate and investigate cellular processes
  • development of peptide-based photosensitizers for the light-induced inactivation of drug-resistant bacteria

  • elucidation of the mechanisms by which cells communicate with one another by sharing material via vesicle secretion and internalization
  • mapping of intracellular protein-protein interactions and impact of viral protein mutations on virus/host responses

Each of these projects involves a combination of chemistry, biochemistry and cell biology. We typically investigate cellular processes at a molecular level and design new tools to study such processes. We use a wide variety of techniques, including protein engineering, peptide chemistry, molecular cloning, and live cell fluorescence microscopy.

Recent Publications

  1. Diaz, J, Pellois, JP. Expressed Protein Ligation: General Experimental Protocols. Methods Mol. Biol. 2020;2133 :75-117.
    doi: 10.1007/978-1-0716-0434-2_5. PubMed PMID:32144664. .

  2. Allen, J, Najjar, K, Erazo-Oliveras, A, Kondow-McConaghy, HM, Brock, DJ, Graham, K et al.. Cytosolic Delivery of Macromolecules in Live Human Cells Using the Combined Endosomal Escape Activities of a Small Molecule and Cell Penetrating Peptides. ACS Chem. Biol. 2019;14 (12):2641-2651.
    doi: 10.1021/acschembio.9b00585. PubMed PMID:31633910. PubMed Central PMC7008471.

  3. Park, G, Brock, DJ, Pellois, JP, Gabbaï, FP. Heavy Pnictogenium Cations as Transmembrane Anion Transporters in Vesicles and Erythrocytes. Chem. 2019;5 (8):2215-2227.
    doi: 10.1016/j.chempr.2019.06.013. PubMed PMID:31482145. PubMed Central PMC6719792.

  4. Arora, M, Ganugula, R, Kumar, N, Kaur, G, Pellois, JP, Garg, P et al.. Next-Generation Noncompetitive Nanosystems Based on Gambogic Acid: In silico Identification of Transferrin Receptor Binding Sites, Regulatory Shelf Stability, and Their Preliminary Safety in Healthy Rodents. ACS Appl Bio Mater. 2019;2 (8):3540-3550.
    doi: 10.1021/acsabm.9b00419. PubMed PMID:31440745. PubMed Central PMC6705617.

  5. Pellois, JP. Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays. Bioessays. 2019;41 (6):e1900031.
    doi: 10.1002/bies.201900031. PubMed PMID:31087674. PubMed Central PMC6563813.

  6. Brock, DJ, Kondow-McConaghy, HM, Hager, EC, Pellois, JP. Endosomal Escape and Cytosolic Penetration of Macromolecules Mediated by Synthetic Delivery Agents. Bioconjug. Chem. 2019;30 (2):293-304.
    doi: 10.1021/acs.bioconjchem.8b00799. PubMed PMID:30462487. PubMed Central PMC6561124.

  7. Fang, Y, Lian, X, Huang, Y, Fu, G, Xiao, Z, Wang, Q et al.. Investigating Subcellular Compartment Targeting Effect of Porous Coordination Cages for Enhancing Cancer Nanotherapy. Small. 2018;14 (47):e1802709.
    doi: 10.1002/smll.201802709. PubMed PMID:30222252. PubMed Central PMC6563816.

  8. Allen, JK, Brock, DJ, Kondow-McConaghy, HM, Pellois, JP. Efficient Delivery of Macromolecules into Human Cells by Improving the Endosomal Escape Activity of Cell-Penetrating Peptides: Lessons Learned from dfTAT and its Analogs. Biomolecules. 2018;8 (3):.
    doi: 10.3390/biom8030050. PubMed PMID:29997347. PubMed Central PMC6165022.

  9. Brock, DJ, Kustigian, L, Jiang, M, Graham, K, Wang, TY, Erazo-Oliveras, A et al.. Efficient cell delivery mediated by lipid-specific endosomal escape of supercharged branched peptides. Traffic. 2018;19 (6):421-435.
    doi: 10.1111/tra.12566. PubMed PMID:29582528. PubMed Central PMC5948172.

  10. Lian, X, Huang, Y, Zhu, Y, Fang, Y, Zhao, R, Joseph, E et al.. Enzyme-MOF Nanoreactor Activates Nontoxic Paracetamol for Cancer Therapy. Angew. Chem. Int. Ed. Engl. 2018;57 (20):5725-5730.
    doi: 10.1002/anie.201801378. PubMed PMID:29536600. PubMed Central PMC6621563.

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