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Michael Manson

Manson, Michael
Michael Manson
Professor of Biology, and of Biochemistry and Biophysics
Office:
BSBE / Room 301A
Email:
Phone:
979-845-5158
Undergraduate Education
B.A. Johns Hopkins University (1969)
Graduate Education
Ph.D. Stanford University (1975)
Postdoc. University of Colorado, Boulder (1975-79)
California Institute of Technology (1980-81)
Joined Texas A&M in 1987

Bacterial Chemotaxis, Motility & Their Use in Biotechnology

The most obvious behavior of Escherichia coli is chemotaxis. Bacteria swim in a 3-D random walk of alternating “runs” and “tumbles.” Cells extend runs towards chemicals they like (attractants) and away from chemicals they do not (repellents). Swimming is propelled by rotation of rigid helical filaments that are driven at their bases by motors powered by a transmembrane proton current. Counterclockwise rotation causes runs, clockwise rotation causes tumbles. We investigate how this tiny (<50 nm diameter) motor assembles from its component proteins and how the proton current couples to rotation. We also study how transmembrane chemoreceptors bind attractants or interact with periplasmic proteins that bind them, how the signal is propagated across the membrane (transmembrane signaling is a function of all cell-surface receptors), and how these signals are integrated and amplified within the cell.

Nanotechnology is a new focus of our group. Starting with chemoreceptors and the functionally related family of transmembrane sensor kinases, we are engineering bacteria to be versatile, efficient, and low-cost detectors of chemicals in the environment. We are also working with mechanical engineers to turn bacteria into miniature beasts of burden to deliver tiny cargo to specified destinations and to harness flagellar motors as system-internal pumps to generate flow in microchannels.

Recent Publications

  1. Jani, S, Seely, AL, Peabody V, GL, Jayaraman, A, Manson, MD. Chemotaxis to self-generated AI-2 promotes biofilm formation in Escherichia coli. Microbiology (Reading, Engl.). 2017; :.
    doi: 10.1099/mic.0.000567. PubMed PMID:29125461. .

  2. Pasupuleti, S, Sule, N, Manson, MD, Jayaraman, A. Conversion of Norepinephrine to 3,4-Dihdroxymandelic Acid in Escherichia coli Requires the QseBC Quorum-Sensing System and the FeaR Transcription Factor. J. Bacteriol. 2017; :.
    doi: 10.1128/JB.00564-17. PubMed PMID:29038253. .

  3. Sule, N, Pasupuleti, S, Kohli, N, Menon, R, Dangott, LJ, Manson, MD et al.. The Norepinephrine Metabolite 3,4-Dihydroxymandelic Acid Is Produced by the Commensal Microbiota and Promotes Chemotaxis and Virulence Gene Expression in Enterohemorrhagic Escherichia coli. Infect. Immun. 2017;85 (10):.
    doi: 10.1128/IAI.00431-17. PubMed PMID:28717028. PubMed Central PMC5607413.

  4. Pasupuleti, S, Sule, N, Cohn, WB, MacKenzie, DS, Jayaraman, A, Manson, MD et al.. Chemotaxis of Escherichia coli to norepinephrine (NE) requires conversion of NE to 3,4-dihydroxymandelic acid. J. Bacteriol. 2014;196 (23):3992-4000.
    doi: 10.1128/JB.02065-14. PubMed PMID:25182492. PubMed Central PMC4248876.

  5. Zhao, X, Zhang, K, Boquoi, T, Hu, B, Motaleb, MA, Miller, KA et al.. Cryoelectron tomography reveals the sequential assembly of bacterial flagella in Borrelia burgdorferi. Proc. Natl. Acad. Sci. U.S.A. 2013;110 (35):14390-5.
    doi: 10.1073/pnas.1308306110. PubMed PMID:23940315. PubMed Central PMC3761569.

  6. Adase, CA, Draheim, RR, Rueda, G, Desai, R, Manson, MD. Residues at the cytoplasmic end of transmembrane helix 2 determine the signal output of the TarEc chemoreceptor. Biochemistry. 2013;52 (16):2729-38.
    doi: 10.1021/bi4002002. PubMed PMID:23495653. .

  7. Liu, J, Hu, B, Morado, DR, Jani, S, Manson, MD, Margolin, W et al.. Molecular architecture of chemoreceptor arrays revealed by cryoelectron tomography of Escherichia coli minicells. Proc. Natl. Acad. Sci. U.S.A. 2012;109 (23):E1481-8.
    doi: 10.1073/pnas.1200781109. PubMed PMID:22556268. PubMed Central PMC3384206.

  8. Adase, CA, Draheim, RR, Manson, MD. The residue composition of the aromatic anchor of the second transmembrane helix determines the signaling properties of the aspartate/maltose chemoreceptor Tar of Escherichia coli. Biochemistry. 2012;51 (9):1925-32.
    doi: 10.1021/bi201555x. PubMed PMID:22339259. .

  9. Manson, MD. Transmembrane signaling is anything but rigid. J. Bacteriol. 2011;193 (19):5059-61.
    doi: 10.1128/JB.05874-11. PubMed PMID:21803989. PubMed Central PMC3187394.

  10. Manson, MD. Not too loose, not too tight--just right. Biphasic control of the Tsr HAMP domain. Mol. Microbiol. 2011;80 (3):573-6.
    doi: 10.1111/j.1365-2958.2011.07578.x. PubMed PMID:21355897. .

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