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Dmitry Kurouski

Kurouski, Dmitry
Dmitry Kurouski
Assistant Professor of Biochemistry and Biophysics
Office:
216 BioBio
Email:
Phone:
979-458-3448
http://www.kurouskilab.com
Undergraduate Education
B.Sc. Belarussian State University
Graduate Education
M.Sc. Belarussian State University (2007)
Ph.D. SUNY at Albany, State University of New York (2012)
Postdoc. Northwestern University (2013-2015)
Joined Texas A&M in 2017

Raman-Based Diagnostics of Plant Diseases

Plant diseases are one of the leading contributors to hunger in the world. controlling disease will play a key role in feeding an estimated 9 billion people by 2050. Disease detection is significant because identifying the cause (be it biotic or abiotic) of plant issues can guide treatment and quarantine. Polymerase chain reaction (PCR) is extremely accurate, but also reagent-hungry, slow, and has limited portability. Our lab uses Raman Spectroscopy to detect structural changes within plant tissues associated with the disease. We analyze our spectra using Multivariate statistical methods to predict the presence of disease. Our objective is to build platforms for Raman disease and stress detection in the field.

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Nanoscale Structural Characterization of Biological Systems Using AFM-IR

The waxy cuticle of many plants acts as a barrier to disease and is an intriguing target for bioengineering renewable polymers. By analyzing intact wax, these properties can be better understood; however, most wax analysis methods involve altering the structure in some way, whether by melting, chemical extraction, or fractionation. Using Atomic Force Microscope Infrared Spectroscopy (AFM-IR), our group is probing intact wax to characterize the distribution of chemical moieties as well as host-pathogen interactions at the surface.

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Plasmonic Catalysis at the Nanoscale

We developed Tip-Enhanced Raman Spectroscopy (TERS) to probe structure and dynamics of biological, photo- and electrocatalytic systems at nanoscale.  In a recent study, we demonstrated that plasmonically active zones of the tip shaft could contribute to the collected Tip-enhanced Raman (TER) signals, which can be an analogy to tip-broadening effect (TBE) in the atomic force microscope (AFM). Moreover, we applied TBE detecting facet-dependent catalytic activity of Au microplates (AuMPs). Our TERS images show the signals of the edge of AuMPs, which was composed of {110} and {100} facets with lower catalytic activity, were dominated by raw species (-NO2), while the reduction mostly took place at the basal part of AuMPs, corresponding to the {111} facet with higher catalytic activity.

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Forensic Analysis of Artificial Hair Dyes

Hair is one of the most common types of physical evidence found at a crime scene. Forensic examination may suggest a connection between a suspect and a crime scene or a victim, or demonstrate an absence of such associations. Therefore, forensic analysis of hair evidence is invaluable to criminal investigations. We develop surface-enhanced Raman spectroscopy (SERS) for confirmatory identification of dyes on hair. Using SERS, we can (1) identify whether hair was artificially dyed or not, (2) determine if permanent or semi-permanent colorants were used, and (3) distinguish the commercial brands that are utilized to dye hair. Expanding upon these results, we continue developing SERS-based dye diagnostics that can be used to solve many important problems in criminalistics and cosmetics.

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Recent Publications

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  1. Morey, R, Ermolenkov, A, Payne, WZ, Scheuring, DC, Koym, JW, Vales, MI et al.. Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy. Anal Bioanal Chem. 2020; :.
    doi: 10.1007/s00216-020-02706-5. PubMed PMID:32451641. .

  2. Kurouski, D, Dazzi, A, Zenobi, R, Centrone, A. Infrared and Raman chemical imaging and spectroscopy at the nanoscale. Chem Soc Rev. 2020; :.
    doi: 10.1039/c8cs00916c. PubMed PMID:32424384. .

  3. Sanchez, L, Baltensperger, D, Kurouski, D. Raman-Based Differentiation of Hemp, Cannabidiol-Rich Hemp, and Cannabis. Anal. Chem. 2020;92 (11):7733-7737.
    doi: 10.1021/acs.analchem.0c00828. PubMed PMID:32401504. .

  4. Farber, C, Sanchez, L, Rizevsky, S, Ermolenkov, A, McCutchen, B, Cason, J et al.. Raman Spectroscopy Enables Non-Invasive Identification of Peanut Genotypes and Value-Added Traits. Sci Rep. 2020;10 (1):7730.
    doi: 10.1038/s41598-020-64730-w. PubMed PMID:32382086. PubMed Central PMC7206150.

  5. Zhou, L, Kurouski, D. Structural Characterization of Individual α-Synuclein Oligomers Formed at Different Stages of Protein Aggregation by Atomic Force Microscopy-Infrared Spectroscopy. Anal. Chem. 2020;92 (10):6806-6810.
    doi: 10.1021/acs.analchem.0c00593. PubMed PMID:32347706. .

  6. Wang, CF, O'Callahan, BT, Kurouski, D, Krayev, A, El-Khoury, PZ. The Prevalence of Anions at Plasmonic Nanojunctions: A Closer Look at p-Nitrothiophenol. J Phys Chem Lett. 2020;11 (10):3809-3814.
    doi: 10.1021/acs.jpclett.0c01006. PubMed PMID:32340455. .

  7. Wang, R, He, Z, Sokolov, AV, Kurouski, D. Gap-Mode Tip-Enhanced Raman Scattering on Au Nanoplates of Varied Thickness. J Phys Chem Lett. 2020;11 (10):3815-3820.
    doi: 10.1021/acs.jpclett.0c01021. PubMed PMID:32340446. .

  8. Sanchez, L, Ermolenkov, A, Tang, XT, Tamborindeguy, C, Kurouski, D. Non-invasive diagnostics of Liberibacter disease on tomatoes using a hand-held Raman spectrometer. Planta. 2020;251 (3):64.
    doi: 10.1007/s00425-020-03359-5. PubMed PMID:32048047. .

  9. Krimmer, M, Farber, C, Kurouski, D. Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer. ACS Omega. 2019;4 (15):16330-16335.
    doi: 10.1021/acsomega.9b01661. PubMed PMID:31616810. PubMed Central PMC6787905.

  10. Wang, R, Kurouski, D. Thermal Reshaping of Gold Microplates: Three Possible Routes and Their Transformation Mechanisms. ACS Appl Mater Interfaces. 2019;11 (44):41813-41820.
    doi: 10.1021/acsami.9b15600. PubMed PMID:31613582. .

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