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Jorge Cruz-Reyes

Cruz-Reyes, Jorge
Jorge Cruz-Reyes
Associate Professor
BioBio / Room 321A
Undergraduate Education
B.S. National University of Mexico (1987)
Graduate Education
M.S. National University of Mexico (1989)
Ph.D. London School of Hygiene and Tropical Medicine (1993)
Postdoc. National Institute of Medical Research, UK (1994)
Postdoc. Johns Hopkins School of Medicine (1995-2001)
Joined Texas A&M in 2001

RNA Editing in African Trypanosomes

The central dogma of Molecular Biology states a linear flow of genetic information: DNA to/from RNA to protein. However, recently discovered processes of RNA editing which modify the genetic material during or after transcription expand this paradigm. A dramatic example is the post-transcriptional maturation of mitochondrial mRNAs by the addition and deletion of U residues in trypanosomes, whereby amino acid coding triplets and stop codons are created. Extensive editing in some mRNAs generates over half their mature size, yet a single error in the U-changes yields a frameshift. Trypanosome RNA editing is developmentally regulated and appears to control mitochondrial respiration.

In vitro studies have revealed that high fidelity RNA editing is directed by small transacting guide RNAs (gRNAs), and is catalyzed by a protein complex or editosome containing endonuclease, exonuclease, terminal transferase and RNA ligase. However, several intriguing questions are still unsolved. For instance, how do gRNA-mRNA interactions modulate these enzymatic activities? How is RNA editing controlled during development? What are the RNA signals and the protein factors involved? We are combining biochemical and genetic approaches to address these questions.

Recent Publications

  1. Cruz-Reyes, J, Mooers, BH, Abu-Adas, Z, Kumar, V, Gulati, S. DEAH-RHA helicaseā€¢Znf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes. RNA Dis. ;3 (2):.
    doi: 10.14800/rd.1336. PubMed PMID:27540585. PubMed Central PMC4987287.

  2. Kumar, V, Madina, BR, Gulati, S, Vashisht, AA, Kanyumbu, C, Pieters, B et al.. REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes. J. Biol. Chem. 2016;291 (11):5753-64.
    doi: 10.1074/jbc.M115.708164. PubMed PMID:26769962. PubMed Central PMC4786712.

  3. Madina, BR, Kumar, V, Mooers, BH, Cruz-Reyes, J. Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing. PLoS ONE. 2015;10 (4):e0123441.
    doi: 10.1371/journal.pone.0123441. PubMed PMID:25928631. PubMed Central PMC4415780.

  4. Madina, BR, Kumar, V, Metz, R, Mooers, BH, Bundschuh, R, Cruz-Reyes, J et al.. Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition. RNA. 2014;20 (7):1142-52.
    doi: 10.1261/rna.044495.114. PubMed PMID:24865612. PubMed Central PMC4114691.

  5. Madina, BR, Kuppan, G, Vashisht, AA, Liang, YH, Downey, KM, Wohlschlegel, JA et al.. Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei. RNA. 2011;17 (10):1821-30.
    doi: 10.1261/rna.2815911. PubMed PMID:21810935. PubMed Central PMC3185915.

  6. Reifur, L, Yu, LE, Cruz-Reyes, J, Vanhartesvelt, M, Koslowsky, DJ. The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing. PLoS ONE. 2010;5 (8):e12235.
    doi: 10.1371/journal.pone.0012235. PubMed PMID:20808932. PubMed Central PMC2923197.

  7. Simpson, L, Aphasizhev, R, Lukes, J, Cruz-Reyes, J. Guide to the nomenclature of kinetoplastid RNA editing: a proposal. Protist. 2010;161 (1):2-6.
    doi: 10.1016/j.protis.2009.10.001. PubMed PMID:19945343. PubMed Central PMC2818556.

  8. Hernandez, A, Madina, BR, Ro, K, Wohlschlegel, JA, Willard, B, Kinter, MT et al.. REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding. J. Biol. Chem. 2010;285 (2):1220-8.
    doi: 10.1074/jbc.M109.051862. PubMed PMID:19850921. PubMed Central PMC2801250.

  9. Hernandez, A, Panigrahi, A, Cifuentes-Rojas, C, Sacharidou, A, Stuart, K, Cruz-Reyes, J et al.. Determinants for association and guide RNA-directed endonuclease cleavage by purified RNA editing complexes from Trypanosoma brucei. J. Mol. Biol. 2008;381 (1):35-48.
    doi: 10.1016/j.jmb.2008.05.003. PubMed PMID:18572190. PubMed Central PMC2596986.

  10. Alatortsev, VS, Cruz-Reyes, J, Zhelonkina, AG, Sollner-Webb, B. Trypanosoma brucei RNA editing: coupled cycles of U deletion reveal processive activity of the editing complex. Mol. Cell. Biol. 2008;28 (7):2437-45.
    doi: 10.1128/MCB.01886-07. PubMed PMID:18227152. PubMed Central PMC2268434.

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