- Undergraduate Education
- B.Sc. University of Guelph, Canada (1981)
- Graduate Education
- M.Sc. University of Guelph, Canada (1983)
- Ph.D. University of California, Davis (1986)
- Postdoc. University of California, Davis School of Medicine (1986-88)
- Joined Texas A&M in 1988
We have explored the ability of plant and marine/fungal species-derived bioactive agents (limonin, curcumin, dietary fibers, n-3 polyunsaturated fatty acids) on mucosal homeostasis, resolution of chronic-Th17 cell mediated inflammation in the intestine, and the suppression of colorectal cancer. Cell culture and transgenic animal models of acute/chronic inflammation and colon cancer have been used in a complementary manner. We have also optimized a primary somatic stem cell colonic crypt “organoid” cell culture system for the purpose of examining the effects of diet-derived chemotherapeutic agents on self-organizing epithelial structure ex vivo. These results provide a critical new paradigm in understanding the molecular mechanisms through which diet modulates, innate immune responses, T-cell activation, intestinal inflammation and colorectal cancer.
By bringing together collaborators in the fields of nutrition, microbiology, pediatrics, computational modeling and statistics, we developed novel “non-invasive” high throughput gene sequencing techniques to simultaneously examine both intestinal gene expression and microbial (microbiome) composition in stool samples. The technique involves isolating genetic material from both intestinal epithelial cells and microbes shed in the subjects’ stools, and comparing the extent to which genes are expressed in both the host and microbiome in subjects administered bioactive dietary agents. This breakthrough technology, for the first time, will provide insight into both host and microbial responses to prebiotic dietary components in human subjects.
Ultimately, the integration of information from the “host” and the microbiome will be used to identify important regulatory pathways of the gut microbiome affecting intestinal development throughout the life span. The use of non-invasive stool-based tests will become critical tools in tailoring diet and practices that modulate epithelial (host) cells and microbiota to promote intestinal development and health.
Torres-Adorno, AM, Vitrac, H, Qi, Y, Tan, L, Levental, KR, Fan, YY et al.. Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux. Oncogene. 2018; :.
Kim, E, Wright, GA, Zoh, RS, Patil, BS, Jayaprakasha, GK, Callaway, ES et al.. Establishment of a multicomponent dietary bioactive human equivalent dose to delete damaged Lgr5+ stem cells using a mouse colon tumor initiation model. Eur. J. Cancer Prev. 2018; :.
Erazo-Oliveras, A, Fuentes, NR, Wright, RC, Chapkin, RS. Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents. Cancer Metastasis Rev. 2018;37 (2-3):519-544.
Fuentes, NR, Mlih, M, Barhoumi, R, Fan, YY, Hardin, P, Steele, TJ et al.. Long-Chain n-3 Fatty Acids Attenuate Oncogenic KRas-Driven Proliferation by Altering Plasma Membrane Nanoscale Proteolipid Composition. Cancer Res. 2018;78 (14):3899-3912.
Triff, K, McLean, MW, Callaway, E, Goldsby, J, Ivanov, I, Chapkin, RS et al.. Dietary fat and fiber interact to uniquely modify global histone post-translational epigenetic programming in a rat colon cancer progression model. Int. J. Cancer. 2018;143 (6):1402-1415.
Jin, UH, Park, H, Li, X, Davidson, LA, Allred, C, Patil, B et al.. Structure-Dependent Modulation of Aryl Hydrocarbon Receptor-Mediated Activities by Flavonoids. Toxicol. Sci. 2018;164 (1):205-217.
Kim, SM, Neuendorff, N, Alaniz, RC, Sun, Y, Chapkin, RS, Earnest, DJ et al.. Shift work cycle-induced alterations of circadian rhythms potentiate the effects of high-fat diet on inflammation and metabolism. FASEB J. 2018;32 (6):3085-3095.
Fan, YY, Fuentes, NR, Hou, TY, Barhoumi, R, Li, XC, Deutz, NEP et al.. Remodelling of primary human CD4+ T cell plasma membrane order by n-3 PUFA. Br. J. Nutr. 2018;119 (2):163-175.
Whitfield-Cargile, CM, Cohen, ND, He, K, Ivanov, I, Goldsby, JS, Chamoun-Emanuelli, A et al.. The non-invasive exfoliated transcriptome (exfoliome) reflects the tissue-level transcriptome in a mouse model of NSAID enteropathy. Sci Rep. 2017;7 (1):14687.
Wei, Q, Lee, JH, Wang, H, Bongmba, OYN, Wu, CS, Pradhan, G et al.. Adiponectin is required for maintaining normal body temperature in a cold environment. BMC Physiol. 2017;17 (1):8.