Department of Biochemistry
University of California, Riverside
Title : “Dynamics of mRNA recognition for translation initiation.”
Recognition of the mRNA cap by protein factors plays a central role in the mechanism of eukaryotic translation initiation. Cap recognition is a complex, dynamic, multi-step, multi-component process that is extensively regulated to control gene expression. Importantly, different mRNAs are recognized with different efficiencies, and for individual mRNAs recognition efficiency can be modulated in response to cellular stimuli. This allows for flexible and rapidly variable translation as befits the needs of the cell, but also contributes to disease when dysregulated. Transcriptome-wide data continue to highlight the complexity and diversity of interactions between mRNA and initiation factors. To probe how these interactions are dynamically coordinated during mRNA recognition, we have developed and applied multicolor single-molecule fluorescence approaches that observe real-time cap binding and initiation factor interactions on full-length mRNAs. Our data offer insights into the molecular basis of mRNA-to-mRNA variability and the role of dynamics in this key component of translational control.
Host: Junjie Zhang
Location: 108 Biochemistry Building (Bldg#1507)