Laurie K. Read
Department of Microbiology & Immunology and Biochemistry
University at Buffalo
Title: “Trypanosome RNA editing: the complexity of getting U in and taking U out”
Expression of most mitochondrial genes in trypanosomatid parasites requires extensive remodeling of mRNAs by Uridine-insertion/deletion (U-indel) editing to create translatable open reading frames. Editing is essential for parasite viability and virulence as edited mRNAs encode proteins involved in mitochondrial bioenergetics. The U-indel editing process entails several hundreds of small, non-coding mRNAs termed guide RNAs, or gRNAs, that direct U insertion and deletion. A dynamic and heterogeneous machinery comprising dozens of proteins organized into several subcomplexes serves as a platform for protein-RNA and protein-protein interactions during editing and catalyzes U insertion and deletion. We use biochemical approaches and a novel bioinformatic platform to understand how the RNA Editing Substrate Binding Complex (RESC) coordinates the U-indel editing in the human parasite, Trypanosoma brucei. I will discuss our studies illuminating distinct functions of RESC proteins the initiation and progression of editing, as well as the roles of two RESC proteins in coordination of this intricate process.
Host: Jorge Cruz-Reyes
Location: 108 Biochemistry Building (Bldg#1507)