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Biochemistry Seminar – Jason Brickner : ” Transcription factors and nuclear pores regulate the spatial organization of the yeast genome to facilitate epigenetic transcriptional regulation.”

March 6 @ 4:00 pm - 5:00 pm

Jason Brickner

Department of Molecular Biosciences

Northwestern University

 

Title: ” Transcription factors and nuclear pores regulate the spatial organization of the yeast genome to facilitate epigenetic transcriptional regulation.”

Abstract:In eukaryotic organisms, many genes physically interact with nuclear pore proteins. In the model system budding yeast, up to 10% of genes physically interact with nuclear pore complexes (NPCs), localize at the nuclear periphery and often show NPC-dependent inter-chromosomal clustering with co-regulated genes. The interaction with the NPC can both promote stronger transcription of active genes and poising of recently-repressed genes (epigenetic transcriptional memory). This interaction requires nuclear pore proteins (Nups) and transcription factors (TFs) and, in the case of memory, chromatin changes. Studying active genes, we identified a conserved “positioning domain” (PD) within the Gcn4 TF that is necessary and sufficient to promote NPC interaction and inter-chromosomal clustering. Inactivation of the PD also leads to a global quantitative defect in transcription of Gcn4 target genes. Studying poised genes, we find that certain repressor proteins can mediate targeting to the NPC and that this interaction alters both chromatin structure and permits Mediator-dependent recruitment of poised preinitiation complex. Poising leads to faster induction and an adaptive fitness advantage based on previous experiences. Finally, to assess the generality of the role of yeast TFs in mediating targeting to the NPC, we performed a global tethering screen. The majority of TFs are sufficient to promote NPC-dependent targeting to the NPCby at least two distinct mechanisms. These results highlight an important and under-appreciated function of TFs in controlling the spatial organization of the gen

Host: Michael Polymenis

Location: 108 Biochemistry Building (Bldg#1507)

Details

Date:
March 6
Time:
4:00 pm - 5:00 pm

Organizer

Michael Polymenis
Email:
polymenis@tamu.edu

Venue

108 Biochemistry Building (Bldg.#1507)
300 Olsen Blvd
College Station, TX 77845 United States
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