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Ry Young

Young, Ry
Ry Young
Professor
Office:
BioBio / Room 311A
Email:
Phone:
979-845-2087
http://young.tamu.edu
Undergraduate Education
A.B. Rice University (1968)
Graduate Education
Ph.D. University of Texas, Dallas (1975)
Postdoc. Harvard Medical School (1975-78)
Joined Texas A&M in 1978

The Molecules and Mechanisms of Bacteriophage Lysis

Most bacterial viruses (phages) cause lysis of their host cell to release the progeny virions. Large phages elaborate an enzyme (“endolysin”) to degrade the cell wall and also a small membrane protein (“holin”). The holin accumulates in the membrane and then, at a precisely scheduled time, suddenly forms a hole to allow release of endolysin through the cytoplasmic membrane to gain access to the wall. We use molecular genetics and biochemistry to study how this small protein is able to act as a molecular “clock” and punch holes in membranes. Small phages make single proteins which cause host lysis in a different way. This strategy is to target the host cell wall synthesis machinery; that is, the virus makes a “protein antibiotic” that causes lysis in the same way as antibiotics like penicillin by inhibiting an enzyme in the multi-step pathway of murein biosynthesis. Thus, when the infected cell tries to divide, it blows up, or lyses, because it can’t make the new cell wall between the daughter cells. Remarkably, each of three different, small phages blocks a different step in the pathway. These small lysis proteins are models for a completely new class of antibacterial antibiotics. Also, the E. coli SlyD protein is required for this mode of lysis in one case. SlyD is a member of an ubiquitous family of proteins related to human “immunophilins,” the targets of immune-suppression drugs. We study SlyD to learn about the role of this class of proteins in biology.

Recent Publications

  1. Cahill, J, Rajaure, M, O'Leary, C, Sloan, J, Marrufo, A, Holt, A et al.. Genetic Analysis of the Lambda Spanins Rz and Rz1: Identification of Functional Domains. G3 (Bethesda). 2017;7 (2):741-753.
    doi: 10.1534/g3.116.037192. PubMed PMID:28040784. PubMed Central PMC5295617.

  2. Gorzelnik, KV, Cui, Z, Reed, CA, Jakana, J, Young, R, Zhang, J et al.. Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ. Proc. Natl. Acad. Sci. U.S.A. 2016;113 (41):11519-11524.
    doi: 10.1073/pnas.1609482113. PubMed PMID:27671640. PubMed Central PMC5068298.

  3. Chen, Y, Young, R. The Last r Locus Unveiled: T4 RIII Is a Cytoplasmic Antiholin. J. Bacteriol. 2016;198 (18):2448-57.
    doi: 10.1128/JB.00294-16. PubMed PMID:27381920. PubMed Central PMC4999926.

  4. Guardia, A, Gulten, G, Fernandez, R, Gómez, J, Wang, F, Convery, M et al.. N-Benzyl-4-((heteroaryl)methyl)benzamides: A New Class of Direct NADH-Dependent 2-trans Enoyl-Acyl Carrier Protein Reductase (InhA) Inhibitors with Antitubercular Activity. ChemMedChem. 2016;11 (7):687-701.
    doi: 10.1002/cmdc.201600020. PubMed PMID:26934341. .

  5. Fan, X, Duan, X, Tong, Y, Huang, Q, Zhou, M, Wang, H et al.. The Global Reciprocal Reprogramming between Mycobacteriophage SWU1 and Mycobacterium Reveals the Molecular Strategy of Subversion and Promotion of Phage Infection. Front Microbiol. 2016;7 :41.
    doi: 10.3389/fmicb.2016.00041. PubMed PMID:26858712. PubMed Central PMC4729954.

  6. Young, R, Gill, JJ. MICROBIOLOGY. Phage therapy redux--What is to be done? Science. 2015;350 (6265):1163-4.
    doi: 10.1126/science.aad6791. PubMed PMID:26785457. .

  7. Liu, M, Gill, JJ, Young, R, Summer, EJ. Bacteriophages of wastewater foaming-associated filamentous Gordonia reduce host levels in raw activated sludge. Sci Rep. 2015;5 :13754.
    doi: 10.1038/srep13754. PubMed PMID:26349678. PubMed Central PMC4563357.

  8. Liu, M, Bischoff, KM, Gill, JJ, Mire-Criscione, MD, Berry, JD, Young, R et al.. Bacteriophage application restores ethanol fermentation characteristics disrupted by Lactobacillus fermentum. Biotechnol Biofuels. 2015;8 :132.
    doi: 10.1186/s13068-015-0325-9. PubMed PMID:26339290. PubMed Central PMC4558781.

  9. Das, M, Bhowmick, TS, Ahern, SJ, Young, R, Gonzalez, CF. Control of Pierce's Disease by Phage. PLoS ONE. 2015;10 (6):e0128902.
    doi: 10.1371/journal.pone.0128902. PubMed PMID:26107261. PubMed Central PMC4479439.

  10. Rajaure, M, Berry, J, Kongari, R, Cahill, J, Young, R. Membrane fusion during phage lysis. Proc. Natl. Acad. Sci. U.S.A. 2015;112 (17):5497-502.
    doi: 10.1073/pnas.1420588112. PubMed PMID:25870259. PubMed Central PMC4418876.

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