- Graduate Education
- M.Sc. Ecole Supérieure de Chimie Physique Electronique de Lyon (1999)
- Ph.D. University of Houston (2002)
- Postdoc. The Rockefeller University (2002-2006)
- Joined Texas A&M in 2006
Protein Chemistry and Cell Biology
Our goal is to determine how proteins function in space and time in the context of complex cellular networks. We focus on chemistry-driven approaches to manipulate protein structure beyond what is feasible with standard genetics. In particular, we use semi-synthetic light-activatable proteins as biophysical probes to investigate protein mechanisms inside living cells. Areas of interest include the important but poorly understood process of protein S-acylation, signal transduction, and protein trafficking.
Najjar, K, Erazo-Oliveras, A, Brock, DJ, Wang, TY, Pellois, JP. An l- to d-Amino Acid Conversion in an Endosomolytic Analog of the Cell-penetrating Peptide TAT Influences Proteolytic Stability, Endocytic Uptake, and Endosomal Escape. J. Biol. Chem. 2017;292 (3):847-861.
Wang, TY, Pellois, JP. Peptide translocation through the plasma membrane of human cells: Can oxidative stress be exploited to gain better intracellular access? Commun Integr Biol. ;9 (4):e1205771.
Robison, AD, Sun, S, Poyton, MF, Johnson, GA, Pellois, JP, Jungwirth, P et al.. Polyarginine Interacts More Strongly and Cooperatively than Polylysine with Phospholipid Bilayers. J Phys Chem B. 2016;120 (35):9287-96.
Erazo-Oliveras, A, Najjar, K, Truong, D, Wang, TY, Brock, DJ, Prater, AR et al.. The Late Endosome and Its Lipid BMP Act as Gateways for Efficient Cytosolic Access of the Delivery Agent dfTAT and Its Macromolecular Cargos. Cell Chem Biol. 2016;23 (5):598-607.
Wang, TY, Sun, Y, Muthukrishnan, N, Erazo-Oliveras, A, Najjar, K, Pellois, JP et al.. Membrane Oxidation Enables the Cytosolic Entry of Polyarginine Cell-penetrating Peptides. J. Biol. Chem. 2016;291 (15):7902-14.
Najjar, K, Erazo-Oliveras, A, Pellois, JP. Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT. J Vis Exp. 2015; (103):.
Li, Z, David, A, Albani, BA, Pellois, JP, Turro, C, Dunbar, KR et al.. Optimizing the electronic properties of photoactive anticancer oxypyridine-bridged dirhodium(II,II) complexes. J. Am. Chem. Soc. 2014;136 (49):17058-70.
Patterson, JL, Arenas-Gamboa, AM, Wang, TY, Hsiao, HC, Howell, DW, Pellois, JP et al.. Materials composed of the Drosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic. J Biomed Mater Res A. 2015;103 (4):1546-53.
Erazo-Oliveras, A, Najjar, K, Dayani, L, Wang, TY, Johnson, GA, Pellois, JP et al.. Protein delivery into live cells by incubation with an endosomolytic agent. Nat. Methods. 2014;11 (8):861-7.
Muthukrishnan, N, Donovan, S, Pellois, JP. The photolytic activity of poly-arginine cell penetrating peptides conjugated to carboxy-tetramethylrhodamine is modulated by arginine residue content and fluorophore conjugation site. Photochem. Photobiol. ;90 (5):1034-42.