- Jorge Cruz-Reyes
- Associate Professor
- BioBio / Room 321A
- Undergraduate Education
- B.S. National University of Mexico (1987)
- Graduate Education
- M.S. National University of Mexico (1989)
- Ph.D. London School of Hygiene and Tropical Medicine (1993)
- Postdoc. National Institute of Medical Research, UK (1994)
- Postdoc. Johns Hopkins School of Medicine (1995-2001)
- Joined Texas A&M in 2001
RNA Editing in African Trypanosomes
The central dogma of Molecular Biology states a linear flow of genetic information: DNA to/from RNA to protein. However, recently discovered processes of RNA editing which modify the genetic material during or after transcription expand this paradigm. A dramatic example is the post-transcriptional maturation of mitochondrial mRNAs by the addition and deletion of U residues in trypanosomes, whereby amino acid coding triplets and stop codons are created. Extensive editing in some mRNAs generates over half their mature size, yet a single error in the U-changes yields a frameshift. Trypanosome RNA editing is developmentally regulated and appears to control mitochondrial respiration.
In vitro studies have revealed that high fidelity RNA editing is directed by small transacting guide RNAs (gRNAs), and is catalyzed by a protein complex or editosome containing endonuclease, exonuclease, terminal transferase and RNA ligase. However, several intriguing questions are still unsolved. For instance, how do gRNA-mRNA interactions modulate these enzymatic activities? How is RNA editing controlled during development? What are the RNA signals and the protein factors involved? We are combining biochemical and genetic approaches to address these questions.
Cruz-Reyes, J, Mooers, BH, Abu-Adas, Z, Kumar, V, Gulati, S. DEAH-RHA helicase•Znf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes. RNA Dis. ;3 (2):.
Kumar, V, Madina, BR, Gulati, S, Vashisht, AA, Kanyumbu, C, Pieters, B et al.. REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes. J. Biol. Chem. 2016;291 (11):5753-64.
Madina, BR, Kumar, V, Mooers, BH, Cruz-Reyes, J. Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing. PLoS ONE. 2015;10 (4):e0123441.
Madina, BR, Kumar, V, Metz, R, Mooers, BH, Bundschuh, R, Cruz-Reyes, J et al.. Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition. RNA. 2014;20 (7):1142-52.
Madina, BR, Kuppan, G, Vashisht, AA, Liang, YH, Downey, KM, Wohlschlegel, JA et al.. Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei. RNA. 2011;17 (10):1821-30.
Reifur, L, Yu, LE, Cruz-Reyes, J, Vanhartesvelt, M, Koslowsky, DJ. The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing. PLoS ONE. 2010;5 (8):e12235.
Simpson, L, Aphasizhev, R, Lukes, J, Cruz-Reyes, J. Guide to the nomenclature of kinetoplastid RNA editing: a proposal. Protist. 2010;161 (1):2-6.
Hernandez, A, Madina, BR, Ro, K, Wohlschlegel, JA, Willard, B, Kinter, MT et al.. REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding. J. Biol. Chem. 2010;285 (2):1220-8.
Hernandez, A, Panigrahi, A, Cifuentes-Rojas, C, Sacharidou, A, Stuart, K, Cruz-Reyes, J et al.. Determinants for association and guide RNA-directed endonuclease cleavage by purified RNA editing complexes from Trypanosoma brucei. J. Mol. Biol. 2008;381 (1):35-48.
Alatortsev, VS, Cruz-Reyes, J, Zhelonkina, AG, Sollner-Webb, B. Trypanosoma brucei RNA editing: coupled cycles of U deletion reveal processive activity of the editing complex. Mol. Cell. Biol. 2008;28 (7):2437-45.